Sentence examples for buffer system with the from inspiring English sources

A cyclometalated iridium complex (1) with indolium block was synthesized successfully and then used as a colorimetric probe for bisulfite in CH3CN-HEPES (v/v, 2 98) buffer system with the detection limit as low as 0.15 μM.

Total RNA was isolated using the XT buffer system with the addition of chloroform/iso-amyl alcohol extraction and LiCl precipitation steps [ 85]. Isolation of total RNA and mRNA purification is described previously [ 39].

Following the general procedure of Wan and Wilkins, RNA was isolated using the XT buffer system with the addition of chloroform/iso-amyl alcohol extractions and LiCl precipitation steps [ 63].

Two buffer systems with the pH range of 3.5 9.0 (15 mM acetic acid, 15 mM Mes and 30 mM Tris) and 5.7 10 (30 mM Aces, 15.6 mM Tris and 15.6 mM ethanolamine) were used.

They suggested exchanging the buffer system with a cheaper phosphate buffer.

Although the VFAs accumulated during the AD, the increased concentration of ammonium formed a buffer system with VFAs, which facilitate growth of certain species in the late phase (50 days), and led to an increase of abundance of diversity (a high number of OTUs, ACE and Chao 1).

To identify glycosylated metabolites, 0.5 µg of purified AA-dihexoside was tested in the appropriate buffer system with 0.1 unit of yeast α-glucosidase (Sigma; 50 mM potassium phosphate pH = 7.5), almond β-glucosidase (Sigma; 50 mM sodium acetate pH = 5.6) or jack bean β-galactosidase (50 mM citrate pH = 4+0.01% BSA), and incubated for 3 hrs at 37°C.

The imaging buffer consisted of a buffer system with an enzymatic oxygen scavenging system containing glucose, glucose oxidase, and catalase to reduce photobleaching, and the thiol to facilitate photoswitching.

We provide a proof of concept that cell metabolism can be exploited to produce in vivo the required co-substrate and co-factor of the reaction by only providing glucose in a buffer system with resting cells.

Results obtained demonstrate the capability of providing service differentiation by using our proposed optical buffer system with multiple thresholds control mechanism.

The recombinant AcCR exhibited 114.4% relative activity to the 4- trimethylsilyl -3-butyn-2-one, and the product e.e. was over 99%, but the yield was only 62.5%, possibly because the substrate could be easily decomposed into a carbonyl alkyne and trimethyl hydroxysilane in buffer system with pH over 6.0 (Zhang et al. 2008).