The same buffer system was used for both buffers and the total buffer volume was kept constant.
Comparing the two buffer systems, we found that 50% more proteins can be extracted from FFPE blocks that were stored for 20 years when the new buffer system is used.
For assembly, a phosphate buffer system was used, which enables the dynamic formation of authentic filaments from soluble tetramers by addition of salt to increase the ionic strength to physiological values [16].
SDS/PAGE was performed essentially as described by Blattler et al. [ 20] except that the Tris/glycine buffer system was used [ 4].
Tricine SDS-PAGE in a discontinuous gel and buffer system was used to estimate the molecular mass of the proteins, under reducing and nonreducing conditions [ 15].
An overlapping 100 mM buffer system was used (pyridine-HCl at pH 4.5, 5.0 and 5.5, and Bis-Tris HCl at pH 5.5, 6.0, 6.5 and 7.0) to determine the pH response of GAD activity.
Various buffer systems were used: NEBuffer 3, pH 7.9, NEBuffer EcoRI, pH 7.5 (both from New England Biolabs), PGM assay buffer, pH 7.0 (see above), and 1 M diethanolamine, 1 mM MgCl2, pH 9.75.
Two different buffer systems were used: Tricine (Fig. 10C) and Laemmli-urea (Fig. 10D).
The gene products of a total of 21 enzyme-coding loci were assayed for electrophoretic variability, the loci scored are given in Additional file 2. Two electrophoretic buffer systems were used.
The following buffer systems were used (50 mM): sodium acetate (pH 5.0), sodium citrate (pH 6.0), sodium phosphate (pH 7.0 and pH 8.0) and sodium carbonate (pH 9.0 and pH 10.0).
