Sentence examples for buffer system consisting of from inspiring English sources

MMP self-activation and activity were prevented by a buffer system consisting of phosphate-buffered saline and 0.05% (vol/vol) Tween 20.

Per reaction, approximately 50 ng of template DNA was used in a buffer system consisting of 10 mM Tris-HCl (pH 9.0), 50 mM KCl, 1.5 mM MgCl2, 0.01% gelatin, 0.1% Triton X-100, 0.2 mM of each of the deoxyribonucleotide triphosphates (Promega Corp ., and 0.2 U Super Taq polymerase (HT Biotechnology Ltd).

For analysis, the hemolymph of Daphnia was considered as a binary buffer system consisting of the carbonate system and a monoprotic non-bicarbonate buffer (HA ↔ H+ + A-).

Briefly, APE1 (50 n) (New England Biolabs) was incubated in a buffer system consisting of 50 m Tris-HCl, pH 8.0, 1 m MgCl2, 50 m NaCl, 2 m DTT at 37°C for 10 min.

Reverse phase chromatography was performed with a binary buffer system consisting of buffer A (0.1% formic acid, 2% acetonitrile in water) and buffer B (0.1% formic acid in acetonitrile).

This column together with a buffer system consisting of water-based mobile buffer A and methanol/acetone-based buffer B, both with a pH between 1 and 2, gave a better peak shape without peak tailing and adequate elution time of 3.7 min (Fig.  1E, ESM Fig.  S1A and S1B).

For HILIC, the buffer system consisted of (A) 25 mM ammonium acetate in water and (B) acetonitrile.

The buffer system consisted of solvent A (20 mM Tris in 6% isopropanol, 4 M urea pH 8.5) and solvent B (20 mM Tris in 6% isopropanol, 4 M urea, 1 M NaCl pH 8.5).

In this case the applied media are buffered by a system consisting of a relatively high amount of NaHCO3 and a 5% CO2 atmosphere to maintain a constant pH between 7.2 and 7.4.

Rotation assays were conducted in buffer C containing an ATP regenerating system consisting of 0.2 mg mL−1 creatine kinase (rabbit muscle, Roche Diagnostics) and 2.5 mM creatine phosphate (Roche Diagnostics).

The fluorescence signal at 370 nm was caused from a blank solution system consisting of methanol:water:phosphate buffer (pH 6.0) (10 10 80, v/v/v).