Sentence examples for buffer supplemented with the from inspiring English sources

Whole-cell lysates were prepared by washing the cells with phosphate buffered saline (PBS) and subjecting them to lysis with Laemmli sample buffer supplemented with the protease inhibitor cocktail.

Proteins are extracted from the still frozen pulverised tissue by addition of Tris-buffered saline to obtain the cytosol fraction of the tumour or by the Tris buffer supplemented with the non-ionic detergent Triton X-100, and, after high-speed centrifugation, are found in the tissue supernatant.

Cells were then washed 3 times in 1x PBS pH 8.0 and lysed in RIPA buffer supplemented with the Complete Protease Inhibitor Cocktail, EDTA-free (Roche).

A 1 10 dilution was produced by adding 1 ml of Butterfields phosphate buffer to 9 ml of Butterfields buffer supplemented with the neutralizing agents.

A 1 10 dilution of each hand rub was produced by adding 1 gram of a hand rub to 9 ml of Butterfields buffer supplemented with the neutralizing agents.

Cells were grown in complete media overnight, treated with dasatinib for 2 48 h, washed, collected by scraping, and lysed in 1% NP-40 buffer supplemented with the appropriate proteinase and phosphatase inhibitors.

PCR reactions were performed with 1 unit of Taq-polymerase (Life Technologies) in the manufacturers' buffer supplemented with 0.2 mM dNTP, in a total volume of 25 μl.

For 22Rv1 RNA sequencing studies, cells were lysed using RLT buffer supplemented with β-mercaptoethanol and processed using the RNeasy mini kit (Qiagen, 74104).

Cells were lysed using RIPA buffer supplemented with protease inhibitors.

All titrations were carried out at 22°C in either G-buffer or G-buffer supplemented with the KCl concentrations indicated and a five-fold molar excess of LatA (Biomol) to actin.

The 900 μl sample was thawed on ice and incubated with the magnetic beads, loaded onto the column and washed with buffers supplemented with the kit.