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Silica HILIC was chosen specifically for optimized phospholipid separation at low buffer strength instead of other commonly used zwitterionic or diol HILIC [ 32, 38].
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The lyticase buffer was used instead of buffer P1.
To avoid streaking on the gels we used 330 µl destreaking buffer (GE Healthcare) instead of rehydration buffer, to which we added 2% IPG-buffer (pI 3 11).
Why, then, not use a buffer solution instead of saline?
Negative control sample was run with methanol/phosphate buffer (1 : 1) instead of tested compound [ 13, 14].
Negative controls were treated with MES-KCl buffer only instead of DAF-2 DA.
Blank sample was run without ABTS using MeOH/phosphate buffer (1 : 1) instead of tested compounds.
Second, ESI MS restricts the type of buffers, buffer strength, and other additives present.
These results clearly demonstrate the effect of the buffer strength on the imaging buffer's acidification behavior.
Control reactions contained storage buffer instead of protein.
For the blank control, the PBS buffer instead of the primary antibody was used.
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