Sentence examples for buffer staining was performed from inspiring English sources

After antigen retrieval in citrate buffer, staining was performed using the BenchMark automated staining system (Ventana Medical System, Tucson, AZ, USA) with primary antibodies against ER-α (SP1, dilution 1∶500), PR (1E2, dilution 1∶500), Ki67 (30-9, dilution 1∶500), c-ErbB2 (4B5, dilution 1∶500), α-enolase affinity purified monoclonal antibodies (mAbs) (ENO-19/8 and ENO-276/3 ENO-276/3l).

After incubation in blocking buffer, antibody staining was performed as per standard procedures.

For detection of GFAP, PFA-fixed and paraffin-embedded CNS sections were incubated with Dako polyclonal rabbit anti-GFAP antibodies (Z0034; 1 13000) in Ventana buffer and staining was performed on a Ventana NexES IHC Slide Stainer using iVIEW DAB Detection Kit (Ventana).

Cells were washed again with Perm/Wash buffer, and intracellular staining was performed using antibodies against CCL4, IL-2, TNF-α, and IFN-γ.

Slides were rinsed three times in wash buffer and subsequent staining was performed at room temperature using a DAKO Autostainer (Carpinteria, CA, USA).

After heat-induced antigen retrieval in 0.01 M citrate buffer (pH6), immunohistochemical staining was performed with antibodies against desmin (Abcam, 1 500), myosin heavy chain slow (Abcam, 1 100), active caspase 3 (Dako, 1 500), CD3 (Dako, 1 250), CD20 (Dako, 1 50) and CD45 (Dako, 1 100).

Postfixation staining of membrane lipids was performed in 1% OsO4 that had been dissolved in 0.1 M sodium cacodylate buffer containing 8% sucrose, staining was performed for 2 hours at room temperature.

Sections were deparaffinized and heated for 20 min at 95°C for antigen retrieval in Tris-EDTA buffer pH 9. TUNEL staining was performed using In Situ Cell Death Detection Kit (Roche, Germany).

Samples were washed twice with 0.1 M sodium cacodylate buffer (pH 7.2) and in-block staining was performed for 16 h with 0.5% uranyl acetate at 4°C.

Intracellular staining was performed with commercial buffers (Cytofix, BD Biosciences; Perm buffer III) for rat CD2, TCR β, phospho-ERK and FoxP3 detection.

Intracellular staining was performed with the eBioscience-staining-buffer-set according to the eBioscience protocol.