Sentence examples for buffer spots were used from inspiring English sources
Crude clarified protein extracts of the E. coli host was used for positive control spots, plain buffer spots were used as negative controls.
Negative controls following either a saturated signal or a buffer spot were used to demonstrate the carry-over of oligonucleotides within print-tip groups in a print-run (this is a frequent problem in microarray printing that is rarely mentioned or quantified) [ 15].
Empty spots and GST spots were used as controls.
PBS buffer, spotted on either side of each protein spot was used to detect protein carry-over during spotting and fewer than 10% of PBS spots showed signal intensities higher than the average nitrocellulose background fluorescence intensity (FI) value (see Materials and Methods).
A specific pattern of buffer spots was positioned to allow calculation of cross-spot carryover during printing.
One hundred fourteen spots with only microarray printing buffer without probes were used as negative controls and were distributed spatially evenly across each array.
Median pixel intensities computed from spot regions were used to represent spot intensity in subsequent analyses.
In Spot Size 2, only individuals with pigment in the wing spot area were used.
In Spot Size 1, the full backcross spot size datasets were used, including individuals with no wing spot pigmentation.
In addition, further negative controls of (i) an amine-linked poly-A fragment (0.1 mg/ml) in 1 × spotting buffer [ 47] and (ii) 1 × microarray spotting buffer alone were included, which were used to monitor any non-specific hybridization.
