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Milli-Q water and all aqueous buffer solutions were sterilized using an autoclave.
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All solutions were sterilized by filtration.
All plasticware, glassware and solutions were sterilized by autoclaving.
The protease solutions were sterilized by filtration through a 0.2-µm membrane.
Stock solutions were sterilized by autoclaving.
The stock solutions were sterilized by filtration through 0.22 μm sterilizing Millipore express filter.
The glucose and MgSO4 solutions were sterilized separately.
All solutions were sterilized by filtration through 0.22 µm filters (Millipore, Molsheim, France) before injection.
Precursor solutions were sterilized with a 0.2 μm nylon syringe filter before gelation (Fisher Scientific, PA).
The solutions were sterilized by filtration through an Acrodisc 0.22 μm filter (Gelman Sciences, Ann Arbor, MI).
All mentioned solutions were sterilized by filtration or autoclaving and flushed with N2 before use.
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