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Buffer solutions were selected according to the requirements of the individual experiments (see below).
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This paper reports on determination of the intrinsic reaction kinetics in reactive extraction of α-cyclohexyl-mandelic acid (α-CHMA) enantiomers with hydroxypropyl-β-cyclodextrin (HP-β-CD) in a modified Lewis cell, in which HP-β-CD dissolved in 0.1 mol/l NaH2PO4/H3PO4 buffer solution was selected as chiral extractant.
Based on physiological environment and the result of preliminary tests, the mixed solution containing phosphate buffered saline solution at pH 7.4 (100 mM) and 150 mM NaCl solution was selected as the release medium for the DNA-PLA-PEG-NPs.
Britton-Robinson buffer/acid/alkaline solution was selected as the support electrolyte to find the optimal pH values for every analyte.
Three different buffer solutions were prepared: pH 4 citrate buffer 0.1 M, pH 6 citrate buffer 0.1 M and PBS 1x (pH 7.4).
Both lipid and buffer solutions were fully degassed before loading into the appropriate cell.
Octanol and buffer solutions were presaturated.
Hence, these buffers were selected in all experiments.
At selected time intervals, buffer solution was taken for UV spectrometer and replaced with fresh buffer solution.
Arteries without any branches were selected and rinsed with sterile solution of phosphate buffer saline (pH 7.4) and 2% antibiotic-antimycotic (ABAM, penicillin-streptomycin; Invitrogen, California).
Three parameters were selected for this study: the buffer pH, the buffer concentration and sodium dodecyl sulphate (SDS) concentrations.
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