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NaH2PO4 and Na2HPO4 of the highest quality used to prepare buffer solutions were purchased from Merck.
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All buffer solution solutes were purchased from Sigma.
Small airway epithelial basal medium (SABM), and small airway epithelial growth media (SAGM™) bulletkit; and Reagentpack™ containing Trypsin 0.025%/EDTA 0.01%, Trypsin neutralizing solution and HEPES buffered-saline solution were purchased from Lonza Walkersvillle Inc. (Walkersville, MD).
For the phosphate buffered saline (PBS), sodium chloride, potassium chloride, 4′,6-diamidino-2-phenylindole (DAPI), and optimal cutting temperature (OCT) cryostat embedding solution were purchased from VWR (Portugal).
RNase A and paraformaldehyde 4% solution were purchased from Affymetrix.
FBS, DMEM and antibiotic/antimycotic solution were purchased from Gibco Life Technologies.
RPMI-1640, penicillin–strepenicillin streptomycinsolution solution were purchased from Gibco (Invitrogen, Karlsolutionermany).
Percoll solution was purchased from Pharmacia, and mixed with 10 × Phosphate Buffer Solution (PBS) with ratio 9 1 to generate 100%% Percoll solution.
Kinetin solution was purchased from Sigma.
Glutaraldehyde (25%% aqueous solution) was purchased from Sinopharm Group Co. , Ltd
Ethidium bromide (EtBr) solution was purchased from Promega (Madison, WI).
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