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Octanol and buffer solutions were presaturated.
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Three different buffer solutions were prepared: pH 4 citrate buffer 0.1 M, pH 6 citrate buffer 0.1 M and PBS 1x (pH 7.4).
Both lipid and buffer solutions were fully degassed before loading into the appropriate cell.
The buffer solution was stored at 4 °C before use.
No sheath flow of buffer solution is needed.
Degradation of sensor stored in buffer solution was evaluated.
At various time points, the enter PBS buffer solution was collected and replaced with 1 ml fresh PBS.
The intrinsic fluorescence quantum efficiency (QE0) of LH2 in aqueous buffer solution is rather low, only 10% (ref. 6).
The buffer solution was phosphate buffer (6 mM, pH 7.4).
A commonly used buffer solution is PBS (phosphate buffered saline solution).
The buffer solution is then collected or re-circulated.
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