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All plasticwares were treated for >2 hours with 0.2 M EDTA to remove the contaminating metals, and all buffer solutions were passed through chelating Sepharose resin and stored in plastic beakers.
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After the buffer solution was passed through for 6.5 h, HSA solution was passed through at 5 mL h-1 for 15 to 16 h.
DNA, in a buffer solution, was passed through a 5.0 μm Cameo 30N filter (Osmonics, Minnetonka, MN) at room temperature before precipitation and then washed with ice-cold 70% ethanol.
K-Hepes (Sigma-Aldrich) buffer, pH 7.4 was added to a final concentration of 100 µM and solutions were passed through an 0.45 µm filter and stored at −20°C; their pH was verified before each experiment.
To separate the small molecule from the probe-DNA, the solution was passed through a NAP5 column using the DNA attachment buffer.
The diluted solution was passed through a column packed with Talon resin pre-equilibrated with the same buffer without imidazole.
Three different buffer solutions were prepared: pH 4 citrate buffer 0.1 M, pH 6 citrate buffer 0.1 M and PBS 1x (pH 7.4).
Both lipid and buffer solutions were fully degassed before loading into the appropriate cell.
Octanol and buffer solutions were presaturated.
Alternating protein and buffer solutions of fixed volume were passed over the substrate until the surface was saturated.
Mcllvaine buffer solution was allowed to slowly pass though the coal bed in 15 minutes.
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