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Insulin concentrations in the high glucose buffer solutions were measured by a rat insulin ELISA kit according to manufacturer's instruction.
Fluorescence intensities of the collected buffer solutions were measured at an excitation and emission wavelength of 633 and 666 nm, respectively (Fluoroskan II, LabSystems, Franklin, MA).
The insulin concentrations in the high glucose buffer solutions were measured by a mouse insulin ELISA kit according to manufacturer's instruction.
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Absorbance of collected DMEM buffer solutions was measured at 370 nm using Shimadzu 1601 UV spectrophotometer (Tokyo, Japan) and compared to a calibration curve to quantify the amount of irinotecan released into the buffer solutions.
The rate constant of ascorbic oxidation of PG electrode in pH 7.2 buffer solution was measured using the linear rotation-scan method.
Because all microbial structures examined herein were suspended in PBS, a sample of this buffer solution was measured to ensure that the contribution from it to the infrared spectra was small.
Metal analysis using USN-ICP-OES The metal content from purified protein sample and the buffer solution was measured using Perkin-Elmer 4300 dual view inductively coupled plasma (ICP) with optical emission spectroscopy (OES) spectrometer, coupled with ultrasonic nebulizer (USN) U-6000 AT, Cetac.
For this reason, fluorescence of the buffers solutions was measured at regular time intervals.
The photoelectrochemical behaviors of TiO2 and BiOCl/TiO2 NTAs in the buffer and glucose solutions were measured by cyclic votammetry and amperometry with different optical powers.
Fluorescence in the conditioned Hanks' buffered salt solution was measured at 485 nm excitation and 535 nm emission.
The water absorption of these saponified graft copolymers in saline and buffer solutions was also measured.
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