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To generate concentration gradients in the microfluidic device, the methanol and buffer solutions were injected with a uniform flow rate (66 μl/min) using a syringe pump (Harvard Apparatus, USA).
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Phosphatidylcholine, cholesterol, and the essential oil of Atractylodes macrocephala Koidz were dissolved in a mixture of scCO2/ethanol, and after the system reached equilibrium, a buffer solution was injected by a syringe pump into the dissolved solutes.
Appropriate volume of sample and/or standard solution containing acetaminophen in pH 2.2 Mcllvaine buffer solution was injected into the proposed FI system and mixed with the flowing stream of supporting electrolyte (pH 2.2 Mcllvaine buffer solution) at an optimum flow rate of 1 ml min−1.
At the next step, 120 μl target solution of various concentrations in the running buffer solution is injected into the measuring flow cell and exposed for 10 min.
At the next step, 120 μl of target solutions of various concentration in the running buffer solution is injected into the measuring flow cell and exposed for 10 min.
If an ETT did not leak, the experiment was terminated at 1 h and a phosphate buffer solution was injected into the space below the cuff and processed as above to determine microleaks.
Approximately 2.5 5 nL of morpholino buffer solution is injected into each egg.
Afterward, cytochrome c in buffer solution was injected into the flowcell, let it incubate for 30 min, and a similar sequence of measurements was applied.
Linearity solutions were injected.
5μL solutions were injected.
Vehicle solutions were injected in the control groups.
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