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Twenty four milliliters of each of the following NaCl TDG buffer solutions was used as an elutant: 0.00, 0.05 (A), 0.10 (B) and 0.20 (C), 0.30 (D), 0.40 (E) and 0.6 (F) mol L−1.
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pH 4.01 and pH 7.01 buffer solutions were used to calibrate the equipment prior to analysis.
To create proper pH environment, standard buffer solutions are used (ISO 8135: 2009, manufacturer JSC "Kyiv Plant RIAP").
The following buffer solutions were used: 50 mM citrate-NaOH buffer (squares, pH 3 6), 50 mM phosphate buffer (triangles, pH 6 8) and 50 mM borate-NaOH buffer (circles, pH 8 10).
In determining the optimum pH and pH range over which the enzyme was stable, the following buffer solutions were used: 50 mM citrate-NaOH buffer (pH 3 6), 50 mM phosphate buffer (pH 6 8), and 50 mM borate-NaOH buffer (pH 8 10).
0.05 mol L−1 Tris-HCl (pH 7.4) buffer solutions are used to maintain the solution pH.
For lactate- or bicarbonate-based CVVH, commercially prepared buffer solutions were used (BH504 or HF32bic respectively, Dirinco, Rosmalen, the Netherlands).
Two buffer solutions were used, the common phosphate buffer PBS and an isotone aqueous solution containing 1.2 g/L of ammonium acetate and adjusted at pH 7. The bacterial layers were formed by depositing 12x5 μl of the bacterial solution onto an area of 1 cm.
A standard Ferrozine/acetate buffer solution was used as a blank.
No buffer solution was used because the buffer ions could potentially reduce the water absorption capacity of the SAP.
After compaction, 1 mg/ml trypsin concentration at pH 7 and with a phosphate buffer solution was used as ultrafiltration solution.
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