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The samples containing varying concentrations of D2O (5, 20, 40, and 60% by volume for the apo and ternary forms; 5, 20, 40, 50, and 60% by volume for the binary form) were prepared by mixing buffer solutions of protonated and deuterated water.
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Buffer solutions of known pH are used to standardize the instrument.
Buffer solutions of 2- N-morpholino)ethanesulfonic acid buffer with a pH of 6.1 and Tris buffer with a pH of 7.8 were prepared freshly before each use.
In order to investigate the effect of buffer components on the reaction, different buffer solutions of pH 8.5 were tested: Clark, Robinson, phosphate, borate and tris buffers.
Testing of pH probe buffer solutions did not detect L.m.
Buffer solution of pH = 2.0 containing 0.3 ?M oxytetracycline.
This is achieved from solutions of PANi protonated with 2-acrylamido-2-methyl-1-propanesulfonic acid in dichloroacetic acid.
In a pH 5.0 buffer solution, Dex BH and Dex CA became protonated and deprotonated, respectively, and self-assembled into well-defined nanospheres.
Why, then, not use a buffer solution instead of saline?
The method is based on direct determination of the ratio of molecular species (protonated) to dissociated (deprotonated species) in a series of non-absorbing buffer solutions.
Molar concentrations of all buffer solutions used were 0.2 M.
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