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Dilutions of the HSA stock with Tris-HCl buffer solution were prepared immediately before use.
The PNGase F enzyme was dissolved in 50 mM AB. NaH2PO4 buffer solution and AB buffer solution were prepared in H2O before dissolving glycopeptides and enzyme, respectively.
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Thiol PEG carboxyl (SH-PEG-COOH) (Mw = 5000 kDa) was purchased from Shanghai Xibao Medpep Co. Phosphate-buffered solution (PBS) (pH 7.4, 0.1 M) as working buffer solution was prepared with 0.1 M Na2HPO4, 0.1 M KH2PO4, and 0.1 M KCl.
Buffer solution was prepared from orthophosphoric acid and its salts in the pH = 7.0.
To this end, a pH-7 buffer solution was prepared of 1.2 g of NaH2PO4 and 0.88 g of Na2HPO4 in 1 L of distilled water.
An aqueous phosphate buffer solution was prepared by dissolving 2.000 g of potassium dihydrogen orthophosphate in approximately 400 mL of water.
To adjust the pH, a phosphate buffer solution was prepared using reagent-grade sodium phosphate monobasic, potassium phosphate, sodium hydroxide, and phosphoric acid.
A phosphate buffer solution was prepared from potassium nitrate monobasic and sodium phosphate dibasic (Helicon, Russian Federation); its concentration was 5 mmol L−1 and pH 6.1 6.2 unless stated otherwise.
The 5.3 pH buffer solution was prepared by dissolving 21.5 g of sodium acetate and 2 mL of acetic acid in 300 mL, before being diluted up to 1 litre with DI water.
To this end, a pH 7 buffer solution was prepared by mixing 1.2 g of NaH2PO4 and 0.885 g of Na2HPO4 in 1 L flask and filled to the mark with distilled water.
Clark and Lubs buffer solution was prepared by mixing 50 ml of 0.2 M aqueous solution of boric acid and potassium chloride (1 liter containing 12.368 g of boric acid and 14.90 g of potassium chloride) with 21.3 ml of 0.2 M sodium hydroxide in 200 ml standard flask [12], and adjusted by pH meter.
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