Sentence examples for buffer solution were loaded from inspiring English sources

For isoelectrofocusing (IEF), 140 μL of sample solution containing around 30 μg total protein, 18.2 mM dithiotreitol (DTT), and 0.5% immobilized pH gradient buffer solution were loaded into 7 cm non linear pH 3 10 strips.

After cross-validation of the biosensing performance of the LSPR platform with the ELISA technique, THP-1 cells suspended in buffer solution were loaded into the LSPR device for on-chip cell separation, incubation, stimulation, and detection of cell-secreted TNF-α.

Then, protein (90 ug/ml) in the buffer solution was loaded into each well for electrophoresis.

400 μL of liposome solution were loaded onto 8 mL G25 gels and washed with different amounts of buffer.

Free CTX at 500, 250, 125, and 62.5 μg/mL was mixed with an equal volume of Laemmli Sample Buffer and the solution was loaded (30 μL) in the precast gel as standards.

To separate free dye from the scFv-bound dye and to exchange the buffer to PBS, the solution was loaded on a microcon concentrator column YM-10 (Millipore) with a membrane-cutoff of 10 kDa.

0.8 g free BCL powder was dissolved in the 5 mL 0.05 M phosphate buffer (pH 7), this solution was loaded into the tube to mix with the MPR.

After 1 10 dilution in 50 mM sodium phosphate loading buffer pH 7.0, the solution was loaded on a 1 mL HiTrap-HIS column (GE Healthcare) that was previously loaded with 1 mL 0.5 M NiSO4 and washed with 10 column volumes of loading buffer.

Pellets were resuspended in 20 µl of 1× NuPage SDS reducing buffer and 10 µl of each solution was loaded on (12%) NuPAGE Novex Bis-Tris Gels 12 wells (Invitrogen, USA) for electrophoresis under constant application of 120 V for 45 min. Electroblotting was performed onto polyvinylidene fluoride membranes (150 V for 1 h).

Sample buffer (Beyotime, China) was added to the cytoplasmic protein and the solution was loaded onto a 6% polyacrylamide gel.

Then, a solution made from 2.0 mL of phosphate buffer (0.2 M, pH 7.4) and 400  μL of the 100  μg/mL KA solution was loaded into a quartz cell.