Before injection, the solution was homogenized by constant stirring.
Then, recently prepared buffer solution was added and the tissues were then homogenized mechanically, sonicated (3 pulses of 10 seconds, 50% amplitude), and finally centrifuged (13000 g, 7′ to 4°C).
The tissues were thawed on ice, the original buffer solution was discarded, new buffer solution was added, and the samples were mechanically homogenized, sonicated (3 pulses, 10 seconds, 50% amplitude), and centrifuged (13000 g, 7° to 4°C).
Weighed brain tissues were homogenized with BHT-Methanol (Sigma, St . Louis MO) and two volumes of chloroform (J.T. Baker, Phillipsburg, NJ) and 0.5 volume of NaH2PO4 (0.2 M -buffer solution were added to the resulting hoM -buffer
Muscle samples were homogenized in buffer solution before centrifugation at 21,880 × g.
The muscle samples were homogenized in buffer solution before centrifugation at 13000 rpm.
Neutrophils were homogenized in buffer solution containing 10 mmol/l Hepes, 1 mmol/l EGTA and 10 mmol/l NaCl2, and then centrifuged at 46,500 g for 20 min at 4°C.
These tissues were homogenized in a buffer solution (100 mg of tissue per mL of RIPA lysis buffer), with the homogenizer immersed in an ice bath.
Briefly, brain tissues were homogenized in phosphate buffer solution (PBS, 20 ml/g of tissue, 0.010 M, pH=7.4).
Briefly, four hemispheres per group were homogenized in phosphate buffer solution (20 ml/g of tissue, 0.010 M, pH=7.4).
