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The samples pretreated with 100 mM ammonium acetate buffer solution were extracted using SPE cartridge.
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The resulting solution was extracted twice with equal volumes of buffer-saturated phenol (Invitrogen, cat. no.
Ringer buffer solution was added (3 mL buffer per g tissue) and the compound was extracted using adaptive focused acoustic ultrasound equipment (Covaris E210, Covaris Inc).
An equivalent volume (50 μl) of buffer solution was added to the blank or control in place of the extract.
The buffer solution was phosphate buffer (6 mM, pH 7.4).
A commonly used buffer solution is PBS (phosphate buffered saline solution).
"A buffer around each tube station was created, and the languages of the local population in each buffer were extracted, to produce a 'second language' (after English) most likely to be spoken by the local community there.
Octanol and buffer solutions were presaturated.
After two washes with phosphate buffered saline (PBS) solution, cells were lysed by using lysis buffer and their proteins were extracted following a commercial extraction method (Pierce Protein Research, Rockford, IL, USA).
After the treatments, soluble intestinal contents from the anterior midgut were extracted in saline solution (0.9% NaCl) and transferred to ice-cold microcentrifuge tubes containing 30 µl of 20 mM HEPES/100 mM NaCl buffer pH 7.4.
Culturable organisms were extracted from sediment samples using a sterile buffer solution prepared by dissolving 0.2% (w/v) sodium pyrophosphate in ultrapure water.
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