After a 30-min GA incubation step, a 1% sodium cyanoborohydride (Sigma-Aldrich, St . Louis MO, USA) in PBS buffer solution was applied, followed by a 30-min incubation step to stabilize the Schiff base bonds formed during GA cross-linking [20].
Tris(hydroxymethyl)aminomethane (THAM) and phosphate buffer solutions were applied to prepare the films that were well deposited to the ITO substrates.
By comparison of two different extraction kits Macherey&Nagel SoilExtract II kit resulted in the highest DNA yields when buffer SL1 and the enhancer solution were applied.
By comparison of two different extraction kits, the Macherey&Nagel SoilExtract II kit resulted in the highest DNA yields when buffer SL1 and the enhancer solution were applied.
Seventy-five micrograms of protein were diluted in 300 μl rehydration buffer and this solution was applied on the strips.
The aggregated PGA was collected by centrifugation (5000 g, 35 min, 4°C), dissolved in 10 mM phosphate buffer (pH 8.0), dialyzed against the same buffer and the enzyme solution was applied to a DEAE-fractogel EMD 6500(S) column.
The precipitate was collected by centrifugation (5000 g, 35 min, 4°C), dissolved in 10 mM phosphate buffer (pH 8.0), dialyzed against the same buffer and the enzyme solution was applied to a DEAE-fractogel EMD 6500(S) column.
After the sample solution was applied, the column was washed with buffer A (50 mM NaH2PO4, 300 mM NaCl, pH 8.0).
The enzyme solution was applied to a hydroxyapatite column equilibrated with 1 mM phosphate buffer (pH 7.0) and PGA was eluted using a linear gradient 0-100% of 200 mM phosphate buffer.
The enzyme solution was applied to a Superdex 200 column equilibrated with 50 mM phosphate buffer (pH 7.0) containing 150 mM NaCl.
