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A further 20 mL of sodium acetate-acetic acid buffer solution were added to achieve pH 5.3, before dilution to 100 mL with water.
EDC of 50 ml and a Na2CO3-NaHCO3 (1:9) buffer solution were added to adjust the pH of the system to approximately 9.
Briefly, 80 μl of sample solution were added into each well of a 96-well microplate, and then a mixture of 100 μl phosphate substrate solution (p-nitrophenyl phosphate) and 20 μl 1.5 M alkali buffer solution were added to each well.
In the next step, 115 μl of the buffer solution were added.
For this reason, 0.01 M of CuCl2 solution, 7.5 mM of ethanol neocuproine solution, and 1.0 M of CH3COONH4 buffer solution were added to each test tube, respectively.
Similar(55)
After 15 min, 100 µl of buffer solution was added and mixed.
After being separated with a NdFeB magnet, 50 nmol of streptavidin in a phosphate buffer solution was added.
250 μL of ALP substrate solution (5 mM pNPP diluted in alkaline buffer solution) was added and the plates incubated for 30 min at 37°C.
To determine exchangeable Ca and Mg, 10 ml of the extract was transferred to an Erlenmeyer flask and 5 ml of an ammonium chloride and ammonium hydroxide buffer solution was added, followed by addition of 1 ml triethanolamine.
10 mL of phosphate buffer solution was added to the settled sludge and shake to disperse the sludge evenly for 10 min, and then the mixed suspension was centrifuged at 4000 rpm for 10 min.
At predetermined time intervals, 5 mL aliquots of the release medium were withdrawn from the release medium, and the same volume of fresh buffer solution was added to continue the release test.
More suggestions(18)
buffer solution were pumped
buffer solution were explored
buffer solution were investigated
buffer solution were taken
buffer solution were studied
buffer solution were perfused
buffer solution were examined
buffer solution were extracted
buffer solution were prepared
buffer solution were loaded
buffer solution were set
buffer solution were incubated
buffer solution were homogenized
buffer solution were recorded
buffer solution were used
buffer solution were applied
buffer solution were captured
buffer solution were obtained
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