Sentence examples for buffer solution was vacuum-infiltrated from inspiring English sources

X-Gluc buffer solution was vacuum-infiltrated into several different tissue samples.

After inoculation period, the root segments were incubated in the staining solution 10 μg/ml WGA AF 488; 0.02% Tween 20 in 1× PBS (137 mM NaCl, 27 mM KCl, 100 mM Na2HPO4, 2 mM K2HPO4, adjusted to pH 7.4) for 60 min; during this period, the solution was vacuum infiltrated three times (3 min each) at 25 mm of Hg.

Fifteen mg of isolated cell wall was vacuum infiltrated under 100 KPa pressure for 5 minutes in 1.25 mL of 50 mM sodium citrate buffer (pH 4.8) followed by addition of 50 μL of 1 mg/mL  T.

Fresh FAA was vacuum infiltrated two additional times.

Subsequently the plant material was vacuum infiltrated for 3 times for 5 min.

Tissues were fixed in 4% (w/v) paraformaldehyde and 0.25% glutaraldehyde in 0.1 M sodium phosphate buffer; samples were vacuum infiltrated for 30 min and then stored overnight at 4°C.

These leaves were vacuum infiltrated into 1 mg/ml fresh DAB solution (pH 3.8) prepared in 10 mM phosphate buffer (pH 7.8) and placed in a plastic box under high humidity and light until brown spots were observed (5 to 6 h).

Four-week-old plants were vacuum infiltrated with suspension of Pst DC3000 or with MgCl2 solution as a mock inoculation control.

Leaf-discs were vacuum infiltrated for 5 min in their respective treatment solutions before they were incubated to various time periods.

Freshly harvested developing fibers (28, 33, and 37 DPA) from TM-1 and im mutant plants were vacuum infiltrated for 5 min with 50 mM potassium phosphate buffer (pH 7.8) containing 0.1% NBT and 10 mM sodium azide and incubated at room temperature for 10, 30, and 60 min.

All samples were vacuum infiltrated for 5 min before the staining process.