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Exact(15)
Thiol PEG carboxyl (SH-PEG-COOH) (Mw = 5000 kDa) was purchased from Shanghai Xibao Medpep Co. Phosphate-buffered solution (PBS) (pH 7.4, 0.1 M) as working buffer solution was prepared with 0.1 M Na2HPO4, 0.1 M KH2PO4, and 0.1 M KCl.
Buffer solution was prepared from orthophosphoric acid and its salts in the pH = 7.0.
To this end, a pH-7 buffer solution was prepared of 1.2 g of NaH2PO4 and 0.88 g of Na2HPO4 in 1 L of distilled water.
To adjust the pH, a phosphate buffer solution was prepared using reagent-grade sodium phosphate monobasic, potassium phosphate, sodium hydroxide, and phosphoric acid.
An aqueous phosphate buffer solution was prepared by dissolving 2.000 g of potassium dihydrogen orthophosphate in approximately 400 mL of water.
A phosphate buffer solution was prepared from potassium nitrate monobasic and sodium phosphate dibasic (Helicon, Russian Federation); its concentration was 5 mmol L−1 and pH 6.1 6.2 unless stated otherwise.
Similar(45)
Dilutions of the HSA stock with Tris-HCl buffer solution were prepared immediately before use.
The PNGase F enzyme was dissolved in 50 mM AB. NaH2PO4 buffer solution and AB buffer solution were prepared in H2O before dissolving glycopeptides and enzyme, respectively.
First, an enzyme-buffer solution was prepared by adding 30 μL of lipase solution (in 10 mM morpholinepropanesulfonic acid (MOPS) and 1 mM EDTA, pH 6.8) to 850 μL of Tris buffer (100 mM Tris HCl and 5 mM CaCl2, pH 7.0).
Three different buffer solutions were prepared: pH 4 citrate buffer 0.1 M, pH 6 citrate buffer 0.1 M and PBS 1x (pH 7.4).
Buffer solutions were prepared from NaCl and HCl of known concentration.
More suggestions(20)
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buffer mixture was prepared
buffer solution was adjusted
buffer solution was added
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buffer solution was drawn
buffer solution was injected
buffer solution was aspirated
buffer solution was obtained
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