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After the buffer solution was passed through for 6.5 h, HSA solution was passed through at 5 mL h-1 for 15 to 16 h.
DNA, in a buffer solution, was passed through a 5.0 μm Cameo 30N filter (Osmonics, Minnetonka, MN) at room temperature before precipitation and then washed with ice-cold 70% ethanol.
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All plasticwares were treated for >2 hours with 0.2 M EDTA to remove the contaminating metals, and all buffer solutions were passed through chelating Sepharose resin and stored in plastic beakers.
To separate the small molecule from the probe-DNA, the solution was passed through a NAP5 column using the DNA attachment buffer.
The diluted solution was passed through a column packed with Talon resin pre-equilibrated with the same buffer without imidazole.
K-Hepes (Sigma-Aldrich) buffer, pH 7.4 was added to a final concentration of 100 µM and solutions were passed through an 0.45 µm filter and stored at −20°C; their pH was verified before each experiment.
Mcllvaine buffer solution was allowed to slowly pass though the coal bed in 15 minutes.
The buffer solution was phosphate buffer (6 mM, pH 7.4).
No sheath flow of buffer solution is needed.
A commonly used buffer solution is PBS (phosphate buffered saline solution).
The buffer solution is then collected or re-circulated.
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