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Sodium carbonate and phosphate buffer solution was obtained from SERVA Electrophoresis GmbH, Germany.
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MEMα (minimum essential medium α), FBS (fetal bovine serum), penicillin/streptomycin and trypsin/EDTA, PBS and Hepes buffer solution were obtained from Invitrogen.
All salts for the preparation of buffer solutions were obtained from Helicon (Moscow, Russia).
The McReynolds compounds (Sigma-Aldrich. Germany), i.e., benzene, 1-butanol, 2-pentanone, nitropropane, and pyridine were of analytical grade, NaCl and the components of buffer solutions were obtained from POCH (Poland).
BSA stock solution was obtained by dissolving the BSA in PBS buffer, physiological pH, with a concentration of 7.6 × 10−6 mol·L−10−6.5 g·L−10−6
Dioctanoyl-PA stock solution was obtained by solubilizing the compound in Tris pH 8 buffer at a concentration of 50 mM.
Afterward, the lysis buffer (1% Triton X-100 in 0.9% NaCl) was added to the cell pellet and mixed until a clear solution was obtained.
Phosphate buffered saline (PBS) of pH 7.2 and 0.25 % trypsin solution were obtained from the company (Thermo Scientific, USA).
All chemicals used for buffers and solutions were obtained from Sigma-Aldrich (Germany) and Merck (Germany) if not mentioned otherwise.
Buffers used to maintain the pH in the drug solutions were obtained from VWR.
SOD working solutions were obtained by dilution in 50 mmol/L phosphate buffer (pH 7.8) and are prepared as needed.
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