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The buffer solution was made of citric acid and disodium hydrogen phosphate with a pH of about 4.8.
A 10% ammonium acetate buffer solution was made by diluting 10 mg of ammonium acetate with deionized water in a 100 ml volumetric flask.
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Enzyme buffer perfusion solution was made up as follows: Fresh aliquots of enzymes were removed from −20°C storage and dissolved in Hank's Balanced Salt Solution (HBSS) (Gibco, Paisley, UK) that had been supplemented with calcium chloride (5 mM) and magnesium chloride (5 mM).
For the protease assay, a 0.3%% azocasein (Sigma-Aldrich) solution was made in buffer B (50 mM Tris HCl, 0.5 mM CaCl2, pH 7.5). 1 mL of filtrate was added to 1 mL of azocasein solution which was then incubated at 37 °C for 15 min.
Working enzyme solution was made (154 μL reaction buffer + 66 μL enzyme per tube assayed).
Because of the instability of STZ in aqueous media, the solution was made using cold citrate buffer (pH 4.5) immediately before administration.
A 20 μmol/l working solution was made using the siRNA suspension buffer provided by the manufacturer.
The aptamer stock solution was made in 40 mM HEPES buffer containing 100 mM NaCl, 5 mM KCl, and 5 mM MgCl2.
The blank solution was made using 0.15 ml of cold extraction buffer to replace 0.15 ml GBSS1 preparation, and the other steps were same as reaction samples.
The blank solution was made using 100 µl of cold extraction buffer to replace 100 µl enzyme preparation and the other steps were same as reaction samples.
Hydrogen peroxide (2 mM) working solution was made by mixing with 50 mM phosphate buffer (pH 7.4).
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