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The buffer solution was kept in motion at 120 rev./min.
Bicarbonate buffer solution was kept in refrigerator and used within about 5 days.
The amount of 0.1 M phosphate buffer solution was kept constant as 10.0 mL during the measurements.
The buffer solution was kept in the refrigerator and left to reach the room temperature before use.
Similar(56)
After centrifugation the sediments were dissolved in a minimal volume of 100 mM Tartarate buffer of pH 3. The solution was kept in a dialysis bag and after sealing securely it was dialyzed against the same buffer.
The solution was kept aside for crystallization.
Volume of solution was kept 50 mL.
This stock solution was kept at -20°C.
During the perfusions, hydrogel solution was kept on ice.
The solution was kept at room temperature for crystallization.
The solution was kept overnight at room temperature.
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