The solution was flushed with acetone for six times and stored in acetone.
The buffer solution was phosphate buffer (6 mM, pH 7.4).
The lid was then removed, and the hot buffer was flushed out with cold water from a running tap.
For the measurements of the low-conductivity MAb1 solution (see Materials), the inside of the aperture tube was flushed with formulation buffer (15 mM histidine hydrochloride, 255 mM sucrose, 0.03% polysorbate 20 at pH 6.0).
After the protein data had been collected, the cell was flushed with buffer and a baseline was recorded.
The segment was flushed with 50 ml of warm Krebs Ringer buffer solution [KRB, pH 7.4, composed of (in mM): NaCl 111.9, KCl 5.0, CaCl21.2, MgCl21.2, NaH2PO40.4, Na2HPO41.6, NaHCO325.0, NaGlutamate 4.9, NaPyruvate 4.9, Na2Fumarate 5.4, glucose 11.5] to remove the luminal contents and then flushed with air to minimize the amount of luminal fluid.
The reaction product was flushed out by injecting a sodium acetate buffer solution (0.1 M, pH 6.8) into the microreactor.
Then, 4 days later, mice were killed and the peritoneal cavity was flushed with 5-ml cold phosphate-buffered solution.
The machine was flushed with egg buffer [ 6] prior to sorting to enhance viability.
Again, the observed inhibition persisted when the reaction chamber was flushed with fresh buffer.
