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Digestion buffer solution was aspirated and replaced with a Ca2+-free enzymatic solution containing 0.1% trypsin, 0.075% collagenase type II, 0.05% cyaluronidase, and 0.025% elastase.
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The enzyme solution was aspirated from the tissues, after which they were rinsed with Hank's buffer and then incubated in Hank's buffer containing DNAse IV (0.1 mg ml-1) for 15 min at 37°C in a water bath.
After each measurement, the fluorescein solution was aspirated and cells were maintained in complete culture medium.
Photosensitizer-containing media solution was aspirated and replaced with fresh culture medium for imaging.
Next, the excess amount of sodium citrate solution was aspirated leaving some to keep the tissue strand hydrated.
After the primary antibody solution was aspirated, the membrane was washed three times with 1 × tris-buffered saline containing Tween 20.
Excess PLO solution was aspirated; then, the surface was rinsed with DPBS before use [27].
Subsequently, the solution was aspirated and the insoluble formazan product was solubilized with acidified iso-propanol.
Eventually, the staining solution was aspirated and traces of staining agent were removed by rinsing several times with PBS.
For electrospinning, the fibroin solution was aspirated with a 2-ml syringe having a diameter of 0.55 mm.
The suspension was centrifuged at 1200 rpm for 6 min, and the sodium citrate solution was aspirated.
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CEO of Professional Science Editing for Scientists @ prosciediting.com