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Mcllvaine buffer solution was allowed to slowly pass though the coal bed in 15 minutes.
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Concentrated hUNG in Buffer B was titrated into a cuvette containing 100 nM of fluorescein labeled DNA in Buffer B. After each addition, the solution was allowed to equilibrate for 4 min inside the fluorometer, and three measurements were averaged.
This solution was allowed to adsorb for 10 min and then washed gently with 200 μl buffer.
The solution was allowed to react at room temperature for 24 h and dialyzed against the phosphate buffer.
Finally, a salting buffer is added over a period of 2 days and the solution is allowed to equilibrate overnight.
Next, 400 μL of Au NPs (0.64 nM) in 20 mM TH buffer solution was added and allowed to react for 5 min. Finally, 8 μL of 10 mM NaCl was added and incubated for another 10 min before being analyzed by the smart-phone device.
The buffer solution was stored at 4 °C before use.
The buffer solution was phosphate buffer (6 mM, pH 7.4).
No sheath flow of buffer solution is needed.
A commonly used buffer solution is PBS (phosphate buffered saline solution).
The buffer solution is then collected or re-circulated.
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CEO of Professional Science Editing for Scientists @ prosciediting.com