Sentence examples for buffer solution was added from inspiring English sources

After 15 min, 100 µl of buffer solution was added and mixed.

After being separated with a NdFeB magnet, 50 nmol of streptavidin in a phosphate buffer solution was added.

250 μL of ALP substrate solution (5 mM pNPP diluted in alkaline buffer solution) was added and the plates incubated for 30 min at 37°C.

To determine exchangeable Ca and Mg, 10 ml of the extract was transferred to an Erlenmeyer flask and 5 ml of an ammonium chloride and ammonium hydroxide buffer solution was added, followed by addition of 1 ml triethanolamine.

10 mL of phosphate buffer solution was added to the settled sludge and shake to disperse the sludge evenly for 10 min, and then the mixed suspension was centrifuged at 4000 rpm for 10 min.

At predetermined time intervals, 5 mL aliquots of the release medium were withdrawn from the release medium, and the same volume of fresh buffer solution was added to continue the release test.

A further 20 mL of sodium acetate-acetic acid buffer solution were added to achieve pH 5.3, before dilution to 100 mL with water.

EDC of 50 ml and a Na2CO3-NaHCO3 (1:9) buffer solution were added to adjust the pH of the system to approximately 9.

Briefly, 80 μl of sample solution were added into each well of a 96-well microplate, and then a mixture of 100 μl phosphate substrate solution (p-nitrophenyl phosphate) and 20 μl 1.5 M alkali buffer solution were added to each well.

In the next step, 115 μl of the buffer solution were added.

For the test, 5 μl of serum or undiluted whole blood and 130 μl of buffer solution are added.