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The SDBS-NPPy/ERGO and SDBS-NPPy films were treated electrochemically in phosphate buffer solution to replace macro SDBS- anions by smaller phosphate anions.
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Many soil testing laboratories use buffer solutions to help estimate lime requirements.
The withdrawn sample was replaced with equal volume of fresh buffer solution to keep the volume of release fluid constant.
Before drug application, to acquire a stable baseline, RA-differentiated SH-SY5Y cells were perfused with buffer solution for 5 min and for 25 min with buffer solution to control, and for 25 min for other drugs dissolved in buffer solution.
At selected time intervals, buffer solution was taken for UV spectrometer and replaced with fresh buffer solution.
The synthetic medium was based upon US CPSC [ 14, 17], artificial saliva but NaCl 150 mM (isotonic solution) and MOPS (no metal binding buffer) were added to replace the PBS buffer in order to prevent precipitation of metal phosphate complexes.
20 After 15 min, one-fifth of the initial volume of the apical compartment (mucosal side) buffer solution was replaced with FITC-labelled dextran (4000 Da, 0.022 g/ml, Sigma).
The blank solution was made using 0.15 ml of cold extraction buffer to replace 0.15 ml GBSS1 preparation, and the other steps were same as reaction samples.
The blank solution was made using 100 µl of cold extraction buffer to replace 100 µl enzyme preparation and the other steps were same as reaction samples.
The blank solution was made using 0.15 ml of cold extraction buffer to replace 0.15 ml of soluble starch synthase preparation, and the other steps were same as reaction samples.
Our solution was to replace the showerhead, rope and all.
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