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Finally, they were washed with buffer solution to remove the unbound protein and excess GA.
The obtained surfaces were washed with buffer solution to remove unbound zeolites before enzyme immobilization.
RNA was washed with buffer solution to remove impurities and eluted in a final volume of 15 μl.
The methanogenic sludge was taken after being acclimated for more than 50 days and rinsed with anaerobic preheated (55°C) buffer solution to remove the residual carbon.
The sediments were centrifuged and rinsed alternately three times with 0.1 M, pH 7.0 phosphate buffer solution to remove non-specifically adsorbed enzymes.
Blood was washed three times with Alserver's buffer solution and one time with bicine-saline buffer solution to remove plasma.
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Then, the cells were washed with phosphate buffered (PBS) solution to remove unbound nanoparticles.
To isolate extracellular bacteria, infected macrophage cultures were washed three times with Hank's buffered salt solution to remove any bacteria that were not inside adherent cells.
Lysis buffer was removed and plugs were rinsed with 50 mM EDTA solution to remove enzymes.
After the membranes were washed with TBS (Tris-buffered solution) buffer to remove the stain, the filters were blocked with 5% nonfat milk in TBS for 1 hour at room temperature, and then incubated with diluted primary antibodies overnight at 4°C.
After incubation, 100 μL aliquots were removed from the buffer solution to be analyzed by UV-Vis spectrophotometry (Infinite M 200 fluorophotometer, TECAN, USA) at λmax of 430 nm.
More suggestions(17)
buffer Ambion to remove
buffer solution to create
buffer solution to deplete
buffer solution to maintain
buffer solution to recover
buffer Qiagen to remove
buffer solution to control
buffer solution to restore
buffer solution to maximize
buffer solution to bring
buffer solution to prepare
buffer solution to replace
buffer solution to measure
buffer solution to reach
buffer solution to investigate
buffer volume to remove
buffer solution to keep
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