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Furthermore, 15 or 5.5 g/L PVP was added to the buffer solution to prepare an isotonic or a hypotonic solution, respectively.
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We then mixed the 54% Iodixanol/Encapsulation Buffer solution with 1x Encapsulation Buffer to prepare equal volumes of 35, 28, 18, and 8% iodixanol/encapsulation buffer.
Figure 1 shows that an acid bearing several acidic hydrogens, such as phosphoric acid, can be used to prepare buffer solutions in several different pH ranges.
Potassium hydrogen phthalate and potassium phosphate monobasic were purchased from EMD and Sigma Aldrich, respectively, in order to prepare buffer solutions.
Before start of the experiment, buffer solutions A and B were prepared according to the following recipes.
Tris(hydroxymethyl)aminomethane (THAM) and phosphate buffer solutions were applied to prepare the films that were well deposited to the ITO substrates.
To this end, a pH-7 buffer solution was prepared of 1.2 g of NaH2PO4 and 0.88 g of Na2HPO4 in 1 L of distilled water.
To adjust the pH, a phosphate buffer solution was prepared using reagent-grade sodium phosphate monobasic, potassium phosphate, sodium hydroxide, and phosphoric acid.
To this end, a pH 7 buffer solution was prepared by mixing 1.2 g of NaH2PO4 and 0.885 g of Na2HPO4 in 1 L flask and filled to the mark with distilled water.
The buffer solution was prepared from KH2PO4 and NaOH (0.1 M) to obtain a pH of 6.5 simulating salivary conditions.
Buffer solution was prepared from orthophosphoric acid and its salts in the pH = 7.0.
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