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The error bars represent the measurement standard error, and measurements were performed 5 min after flush-in of the buffer solution to deplete any initial oxygen concentration.
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Cytoplasmic Na+ diffusivity (DNa) was measured in myocytes equilibrated initially in Na+-free/Ca2+-free solution to deplete intracellular Na+ without causing Ca2+ overload via reverse mode NCX.
Results were read 20 minutes after adding the buffer solution to the sera.
The dried lipids are solubilized in phosphate buffer solution to form a multilamellar vesicle (MLV) solution.
Blood was washed three times with Alserver's buffer solution and one time with bicine-saline buffer solution to remove plasma.
Each drug solution in these master plates was diluted with phosphate buffer solution to produce 500 µM pre-diluted plates.
An aliquot of each sample or standard solution was stained with bromocresol green buffer solution to measure total alkaloids content.
ABTSº+ solution was diluted with phosphate buffer solution to absorbance values of 0.70 ± 0.02 at 734 nm.
Lysozyme was added to the buffer solution to reach a concentration of 100 g/L.
At the desired time points, hydrogels were removed from the buffer, weighed, and returned to buffer solution.
Weekly, samples were transferred to buffer solution and stored at 4°C.
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