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The saturation of the adsorbent sites may also have been associated with the presence of a phosphate buffer solution to control the medium pH, especially in the case of OSP.
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Many metal (or metalloid) species are water soluble, and they can be extracted with water or buffer solution necessary to control the pH and prevent degradation of coordination complexes.
This control was lacking in the Alvarez-Alfageme et al. [8] studies as well as the addition of buffer solution to the control.
The radiometal, ligand, and buffer solutions were controlled to mix in the chip with different volume ratio using LabVIEW interface software.
We also discuss methods to control the appropriate SiO2 thickness and the refractive index of the buffer solution to maximize measurement sensitivity.
The dried lipids are solubilized in phosphate buffer solution to form a multilamellar vesicle (MLV) solution.
The pH of the phosphate and acetate buffer solutions was controlled by a digital pH metre (InoLab pH/ion, WTW GmbH & Co. KG, www.wtw.com) calibrated at 25 °C with standard buffers of pH 7.0 and 4.0 (WTW GmbH & Co. KG, www.wtw.com).
For APTT assay, 100 μL citrated plasma was mixed with 100 μL of test compound or buffer solution for test and control reactions respectively and incubated at 37°C for 1 minute; 200 μL of APTT reagent was then added and the mixture was again incubated at 37°C for 4 minutes.
Enzymatic hydrolysis was performed in 0.05 M sodium acetate buffer solution (pH 4.80) to control the same pH.
During the total preparative procedure, NH4OH acts a pH buffer to control the pH value of the solution and slow donate of OH– ions.
The homogeneous application of trypsin by a spraying device can overcome this problem if spraying conditions such as flow rate, distance and buffer solution are carefully controlled.
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