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After prolamine extraction with ethanol 60%, samples were serially diluted (1 10 1 20,000) in the buffer solution provided by the manufacturer for concentrations that corresponded to 6, 25, 50, 100, 250, and 500 ppm.
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DNA was eluted twice in 50 μl of the buffer solution provided with the kit.
Two drops of the chase buffer solution provided in the kit were added to the test tube.
Blood samples were lysed within one hour of being drawn, diluting 1 mL of whole blood with 1 mL of phosphate buffered saline (PBS) and adding 2 mL of lysis solution provided by the manufacturer.
The next day, the plate was washed three times with EIA buffer (250 μl per well) and 250 μl of diluted blocking solution (provided by the manufacturer) was added to each well.
The solution provided by [25] scavenges ambient energy from AM band and buffers it for further use by structural health monitoring wireless sensor.
Global problems need global solutions provided by global coalitions.
Storage buffer and HNT-PBS solution were provided by RnAssays (Utrecht, the Netherlands).
A 20 μmol/l working solution was made using the siRNA suspension buffer provided by the manufacturer.
In another experiment, mice were administered MTP-131 as an ophthalmic-formulated solution (Ocuvia, provided by Stealth Peptides) daily via eye drops [in 0.01 M sodium acetate buffer solution (pH 6.00); 5 μl/eye] or with buffer alone.
Electrophoresis was performed in alkaline buffer (pH 9.4) provided by the manufacturer (Sebia), with separation primarily due to the pH of the solution and endosmosis.
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