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The EK process is designed by a buffer solution of sodium acetate and a working-solution circulation system to neutralize the pH of the soil matrix.
The values of liquid side mass transfer coefficient for the multifilament wire gauze packings were obtained by absorbing lean carbon dioxide in a buffer solution of sodium carbonate and sodium bicarbonate.
Liquid side mass transfer in cocurrent upward gas-liquid flow in empty tubes of three different sizes, viz. 10, 15 and 20 mm has been studied by absorbing carbon dioxide from a mixture with air into a buffer solution of sodium carbonate and sodium-bicarbonate.
The reaction was carried out in a batch reactor at pressures between 1.5 to 2.5×103 kPa and temperatures of 333 and 343 K. Ethanol was added as a cosolvent to enhance the octene solubility in the aqueous phase and a buffer solution of sodium carbonate and bicarbonate was used to eliminate the formation of acetals.
A solution of OTC (oxytetracycline hydrochloride, HPLC grade ≥ 95%% purity, Sigma-Aldrich) was previously prepared at 240 mg L−1 (stock-solution) in a buffer solution of sodium acetate (Synth)/acetic acid (Synth) at 0.1 mol L−1 in ultrapure water (Milli-Q).
Unless indicated, the pH of the buffer solution of sodium acetate was 4.8.
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A buffer solution of 0.01% sodium azide (Sigma Chemical Co., USA) was added to prevent microorganism contamination.
The substrate peptide, Arg-Val-Nle- pNO2-Phe -Glu-Ala-Nle-NH2, wArg-Val-Nle- pNO2-Phe -Glu-Ala-Nle-NH20 mM sodium Arg-Val-Nle- pNO2-Phe -Glu-Ala-Nle-NH2
For dosing, we prepared 100 ppm MeHg stock solution weekly by dissolving crystalline CH3HgCl (Alfa Aesar, Ward Hill, MA) in a buffer solution of 5 mM sodium carbonate (Na2CO3; Mallinckrodt & Baker, Inc., Phillipsburg, NJ).
The meals were prepared as follows: a buffer solution of 200 ml sodium phosphate (20 mM; pH 5.5) was heated to 100°C, a 25-ml starch solution containing 10 g starch from potato (Sigma), was added and the mixture was cooled down to 37°C.
Briefly, ∼3 mg of - anti-DB[ a, l]PDE was dissolved in 350 μL of THF and added to an 8 mg buffer solution of 5′-CCATCGCTACC-3′ [25 mM sodium acetate, 25 mM Tris, and 25 mM TEAA (pH 11)].
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