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Why, then, not use a buffer solution instead of saline?
Control embryos were incubated in Hanks' buffer solution instead of primary antibody and then treated with complement.
Before and after incubation, the absorbance was measured at 405 nm and compared to the control that contained 25 μl of buffer solution instead of plant extract.
The reaction mixtures were incubated at 25°C for 5 min. Before and after incubation absorbance readings were recorded at 405 nm using a micro-plate reader (Thermomax, Molecular device Co., Virginia, USA) and compared to a control which contained 50 μl of the buffer solution instead of the extract.
The inhibition activity was calculated using the following equation: Inhibition activity = [(A − B)/ A] × 100%% where A is the reaction blank, of which the mixture contained the same volume of the buffer solution instead of the ACE inhibitor sample; B is the reaction in the presence of both ACE and its inhibitor.
Similar(55)
Modified M56 buffer without chlorine was also tested as the electrolyte solution instead of NaCl buffer or LB medium.
A negative control of each section was performed using PBS (phosphate buffer solution, pH8) instead of the primary antibody.
They can be the solution instead of the end game.
Some of these guys want to be the solution instead of part of the solution.
Ca2+-free solution instead of CaCl2 contained 0.5 mM EGTA.
Therefore, quartimin results in just one rotation solution, instead of multiple solutions.
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