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Initially, a test sample plug is loaded into a capillary filled with buffer solution followed by N36 peptide solution, and the two solutions simultaneously mix by diffusion.
The supernatant was collected and evaporated under a stream of nitrogen at 50 °C, after which the residue was dissolved in 10 mL HAc-NaAc buffer solution, followed by sonication for 5 min.
After HCl incubation, the sections were rinsed with Borate buffer solution followed by PBS rinses.
Control groups either received tumor cells followed by a sham-operation or were injected with a buffer solution followed by PH.
Later, DCFH-DA was removed, and the cells were washed with 1 × buffer solution followed by PDT treatment with different concentrations of HY.
After HCl incubation, sections were rinsed with Borate buffer solution followed by PBS rinses, incubated with 0.3 M glycine in 0.4% Triton X-100 (PBS-T) for 30 min, and rinsed.
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This step was repeated with 300 μL of wash buffer working solution followed by centrifugation at 13 000 xg for 1 min in order to dry the filter fleece completely.
A small fragment of the gastric wall from each animal was fixed in 10% buffered formalin solution followed by tissue dehydrated with alcohol and xylene.
Heart cryosections, sectioned in slices of 7 µm, were air-dried and fixated in 4 %-saline buffered formaldehyde solution, followed by incubation with Oil-Red-O (Fluka; Sigma-Aldrich) for 30 min.
The DNA modified electrode was incubated in Co phen 33+ solution of an aqueous buffer or an acetonitrile (AN) solution, then it was rinsed and placed in a Co phen 33+ free buffer solution or AN solution, followed by cyclic voltammetric experiments.
Sample fluorescence for each cell type was normalized to the fluorescence obtained from 0.25×105 cells plated directly in triplicate wells of a 24 well plate, allowed to adhere for 6 hours at 37°C, 5% CO2, and then quantified by the same method (incubation with Cell Dissociation Buffer and 4x Lysis+Dye Solution followed by one round of freeze-thaw lysis).
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