Sentence examples for buffer solution consisting of from inspiring English sources
A pH 7.4 phosphate buffer solution (consisting of 0.04 M KH2PO4/NaH2PO4, 0.25 M sucrose and 0.15 M KCl) was used with rat liver tissue at 1 4 w/v and liver tissue was homogenized using an OMNI homogenizer, followed by centrifugation of the homogenate at 10,000g for 22 min at 4 °C.
After hydrolysis, the reactor was cooled to 40 °C and 1.3 ml 25%% aqueous ammonia was added, followed by 1.3 ml buffer solution consisting of 1 M ammonium dihydrogen phosphate and 1 M diammonium hydrogenphosphate to neutralize the solution prior HPLC purification.
The isocratic mobile phase was composed of a buffer solution consisting of 25 mM potassium phosphate monobasic in water containing 0.25% triethylamine and acetonitrile (35:65% v/v).
Passage 2 (P2) and passage 4 (P4) ASCs that had been cultured in all six media types were suspended in a buffer solution consisting of PBS with 2% FBS.
The supernatant of each sample was discarded and the resulting pellet was added to a buffer solution consisting of 0.5% hexadecyltrimethylammonium bromide dissolved in 50 mM potassium phosphate buffer, pH 6, containing 50 μl of protease and phosphatase inhibitor cocktails.
Next, 40-μL solution of PTP1B or VHR was dissolved in a buffer solution consisting of 0.06 M citric acid (pH 6.0), 0.1 M NaCl, 1 mM EDTA and 1 mM DTT.
Phosphate buffer solution consists of phosphate, carbonate and chloride while 0.5 mol/L NaCl contains only chloride.
The NMR buffer solution consisted of 10 mM Na2HPO4 and 100 mM NaCl (pH 6.8), and 25 nM DSS (2,2-dimethyl-2-silapentane-5-sulfonate 2,2-dimethyl-2-silapentane-5-sulfonate 2,2-dimethyl-2-silapentane-5-sulfonate 2,2-dimethyl-2-silapentane-5-sulfonate 2,2-dimethyl-2-silapentane-5-sulfonate 2,2-dimethyl-2-silapentane-5-sulfonate
The Tris buffer solution consists of 15 mM Tris(hydroxymethyl)aminomethane, 1.25 mM NaH2PO4, 2.0 mM Na2SO4, 3.25 mM KCl, 140 mM NaCl, 1.2 mM CaCl2 dehydrate, and 1.2 mM MgCl2 hexahydrate at pH 7.4 (all Fisher, Suwanee, GA).
Protein buffer solutions consisted of 25 mM potassium phosphate buffer, 25 mM NaCl, 5% (v/v) D2O, 0.02% (w/v) sodium azide, pH 7.0.
Among the various tested loading buffer conditions, a solution consisting of 200 mM glycolic acid, 50% acetonitrile, and 1% trifluoroacetic acid was optimal for high selectivity and recovery (data not shown).
