The cranium and dura mater were divided sagitally and each half was placed in a buffer solution composed of NaCl 119 mM, NaHCO3 15 mM, KCl 4.6 mM, MgCl2 1.2 mM, NaH2PO4 1.2 mM, CaCl2 1.5 mM and glucose 5.5 mM.
The thermosensitive behavior of the semi-IPN gel was investigated in a buffer solution composed of a relatively high concentration of sodium chloride and sodium citrate as salts, and sodium dodecyl sulfate as a surfactant, which are generally used as a buffer solution in biochips.
For this purpose, buffer solutions composed of a mixture of NaF and HClO4 are used.
To obtain a fraction enriched in postsynaptic membranes (PSD), the protein amount was calculated in the pure synaptosomal fraction, and a 4 mg/ml solution was prepared with buffer B. An equal volume of a solution composed of Triton X-100, 0.5 mM Hepes/KOH, and protease inhibitors was added and stirred for 15 min on ice.
After DCM addition, the population fluorescence was monitored for a further 10 min. For each strain, a standard curve was constructed by diluting the DCM-grown culture to a final OD600 of 0.01 in a solution composed of 30 mM buffer, 50 mM NaCl, 3 mM KCl, 10 µM valinomycin, and 10 µM nigericin.
Maximum GUS specific activity (9.5 × 103 U/mg) with high total activity recovery (8.9 × 104 U/mL) was obtained using a simple extraction solution composed of 50 mmol/L citrate buffer at pH 5.25.
Briefly, 0.200 g of freeze-dried sludge is transferred to a 16 × 150 mm borosilicate glass screw-top conical tube before adding 8 mL of a solution composed of methanol / 0.1 M acetic acid buffer solution pH 4.0 (1:1 v/v).
Under profound barbiturate anesthesia (pentobarbital, Ceva santé animale, 150 mg/kg, i.p ., rats were transcardially perfused with Ringer's lactate solution containing 0.1% heparin followed by 500 ml of a fixative solution composed of 4% paraformaldehyde in 0.1 M phosphate buffer (PB, pH 7.4).
The culture samples were immersed in a fixative solution composed of 1% glutaraldehyde in 0.1 M potassium buffer (pH 6.8), at room temperature for 16 h.
Males were then injected intraperitoneally on each side of the body with approximately 100 µL of a fluorescein dye solution composed of 1 mg/ml fluorescein in physiologically buffered saline (PBS).
After washing with Tris buffer and dehydration with acetonitrile the gel cubes were soaked with trypsin solution composed of 10 ng/ml trypsin (Promega) in 20 mM Tris/HCl pH 8, 0.01% ProteaseMax (Promega) for 30 min on ice.
