Sentence examples for buffer slides were incubated from inspiring English sources

After 6 washes in blocking buffer, slides were incubated with donkey anti-rabbit affinity purified Cy3-conjugated IgG antibodies.

After 6 washes in blocking buffer, slides were incubated with secondary antibodies.

After washing in Tris buffer, slides were incubated for 2 h with the primary antibody (COX-2, Cayman, USA, dilution 1 : 100).

After three more washes in Dako buffer, slides were incubated with chromogen 3,3′-diaminobenzidine tetrahydrochloride (DAB), counterstained with hematoxylin, and mounted.

After rinsing in wash buffer, slides were incubated overnight at 4°C with the anti-phospho-EGFR Tyr-11733) monoclonalclonantibodyoDAKOdiluteddiluted 1 : 100.

After the application of an equilibration buffer, slides were incubated in working strength TdT enzymes that contained dUTP-digoxigenin for 1 h (at 37°C).

Primary antibodies were diluted in blocking buffer and slides were incubated for 20 minutes at 37°C, followed by overnight incubation at 4°C extended by 20 minutes at 37°C.

After washings with Rince Buffer Biogenex, slides were incubated with the secondary anti-fluorescein-AP conjugate, and the signal was revealed with Fast Red substrate solution for 20 min. Slides were lightly counterstained with hematoxylin prior to aqueous mounting by Aqua-Perm (Shandon Aqua-Perm™ Thermo Electron IVDD Compliant, Waltham, MA).

After rinsing in wash buffer, the slides were incubated with HRP conjugated appropriate secondary antibody.

After two rinses in buffer, the slides were incubated with the detection system for 30 min (labeled polymer-HRP).

After heating in citrate buffer, the slides were incubated with primary antibodies overnight at 4 °C and then blocked with peroxide.