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For data analysis, all diffraction images were azimuthally integrated, averaged and the buffer signal was subtracted from that of the buffered protein solution.
In general, a mAb is considered polyol-responsive if the signal drops to 50% of the TE buffer signal when incubated with polyol buffer.
After incubation for 1 hour at room temperature with an HRP-conjugated anti-mouse secondary antibody (1∶5000 in blocking buffer), signal was detected using ECL femto reagent (Thermo Scientific, Rockford, USA) and visualized with a BioRad ChemiDoc imaging station and glycotpyes were evaluated using published protocols [41].
The spectra were averaged over two scans and corrected by subtraction of the buffer signal.
Following 2 days of rinsing in MABT and alkaline phosphatase buffer, signal was developed in BM Purple (Roche).
Raw data from three measurements were averaged, corrected for buffer signal, smoothed and converted to mean residue ellipticity.
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Water and buffer signals served as blank background signals.
Hence, miRNAs balance and buffer signaling events, crucial for proper tissue homeostasis.
After thorough washing the membranes with TBST buffer, signals were developed with an enhanced chemiluminescent system (Pierce).
The frequently used 60 Hz refresh rate, for instance, results in frames of 16.7 ms. These CRT characteristics determine how CRT monitors can be controlled: The graphics adapter of the computer needs to be synchronized to the vertical blank of the monitor during which the buffered signal of the graphics card is sent.
These engineered proteins include ion channel subunits, optogenetic effectors, intracellular ionic buffers, signaling enzymes, membrane-tethered neuropeptides, and membrane-tethered peptide neurotoxins that target specific ion channel subtypes.
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