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Unlike other techniques such as transient isotachophoresis and temperature gradient focusing, this method does not require multiple buffer setup or temperature sensitive buffer.
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The samples are sent from the source where they accumulate in an IFFT setup buffer.
Fender Bassman setup.
Unlike other MAC protocols, supporting construction of multi-hop flows, CL-MAC considers all pending packets in the routing layer buffer and all flow setup requests from neighbors, when setting up a flow.
Norman [20] explored a flowshop scheduling problem with finite buffer and sequence-dependent setup times and proposed a TS method.
Specifically, a policy that can deliver good and stable performance resilient to variations in system parameters (such as buffer sizes, processing rates, and setup times) is viewed as a "robust" policy.
We used published aDNA protocols at McMaster, including the use of dedicated clean-room facilities for sample and buffer preparation, DNA extraction, PCR setup, and post-PCR work.
The buffer preparation, DNA extraction and setup of PCR were done in a second room, which had a separate PCR preparation hood (PCR-Workstation, peQLab, Biotechnologie GmbH).
Instead, a number of fixed timeslots can be setup so that "buffered" tweets go out to followers at specific times of the day.
The setup for the photonic buffer is schematically shown in Fig. 1c) (a detailed description of the setup can be found in the 'Methods' section and in Supplementary Fig. 1).
The validation for parallel triplex formation was conducted in sodium acetate buffer, pH 4.8, using the same setup as described for antiparallel duplex formation.
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