DNA was purified using the QIAGEN® Genomic-tip 100/G columns and QIAGEN® Genomic DNA Buffer Set.
If cred_handle is not a proxy credential, data_set will be set to an empty buffer set.
Intracellular staining of Foxp3 was conducted by using the Foxp3/transcription factor staining buffer set, according to the instructions provided by the manufacturer (eBioscience).
Best quality results (DNA and RNA integrity conservation, ratios A260/280 and A260/230) were obtained with NucleoSpin RNA kit combined with DNA elution buffer set.
If the call succeeds and cred_handle is a proxy credential, data_set will be set to a single-element buffer set containing the unparsed principal name of the intermediate service.
Then, three simultaneous DNA /RNA extraction protocols were compared: Trizol method followed by RNeasy purification kit (Qiagen, Hilden, Germany), NucleoSpin RNA kit combined with DNA elution buffer set (Macherey-Nagel), and Nucleobond kit (Macherey-Nagel).
where U represents a full buffer set.
The pH stability of ScLac was investigated by incubating an aliquot of the purified enzyme preparation in the same buffer set used to determine the pH optima for 0, 1, 6 and 24 h (Fig. 4a).
Endotoxins were removed in an additional step (Endofree buffer set; Qiagen, Santa Clarita, CA).
Foxp3 staining was performed, after staining of CD3, CD4 and CD25 surface antigens, using the Foxp3 buffer set from eBioscience according to the manufacturer instructions.
