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Fixable Viability Dye and FOXP3 staining buffer sets were purchased from eBioscience.
Intranuclear Foxp3 staining was conducted with the BD or eBiosciences Transcription Factor Buffer sets according to the manufacturers' protocol.
Dot blot analysis was undertaken with the use of the DIG DNA Labeling and Detection Kit, Dig Easy Hyb, and DIG Wash and Block Buffer Sets from Roche.
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where U represents a full buffer set.
To detect intracellular Bcl6, cells were stained in the Foxp3/Transcription Factor Staining Buffer Set (eBioscience) after surface staining.
Among many others mentioned in the previous section, the work in [48] considers opportunistic scheduling in a setting where users' queues are infinitely backlogged (this full buffer setting is typically used to model elastic or best effort flows).
The pH stability of ScLac was investigated by incubating an aliquot of the purified enzyme preparation in the same buffer set used to determine the pH optima for 0, 1, 6 and 24 h (Fig. 4a).
Endotoxins were removed in an additional step (Endofree buffer set; Qiagen, Santa Clarita, CA).
After that cells were washed and stained using a permeabilisation buffer (Foxp3 Staining Buffer Set, e-Biosciences).
The Strep purification was done using the Strep-tag® Protein Purification Buffer Set and according to manufactures protocol (IBA).
Intracellular FOXP3 staining was performed using the FOXP3 Fix/Perm Buffer set (Biolegend) according to the manufacturer's recommendations.
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