Sentence examples for buffer separated on a from inspiring English sources

Samples were mixed with 2X SDS-PAGE loading buffer, separated on a 12% polyacrylamide gel and transferred to nitrocellulose membrane.

Fifty micrograms of the lysate protein was boiled in a Lammli buffer, separated on a 10% SDS polyacrylamide gel, and then transferred to a nitrocellulose membrane.

Protein extracts were dissolved by boiling in SDS-loading buffer, separated on a 12% SDS-polyacrylamide gel, transferred to a PVDF membrane, and immunodetected.

The bound proteins were eluted from beads by boiling in the LDS sample buffer, separated on a 4 12% Bis-Tris gel and visualized by silver staining.

FLAG- Nab3 was immunoprecipitated with anti-FLAG M2 Affinity Gel (Sigma-Aldrich, A2220), eluted in SDS sample buffer, separated on a SDS-polyacrylamide gel, and immunoblotted with either anti-FLAG or anti-acetyl lysine.

The exosome-rich pellet and a human hepatocellular carcinoma Huh-7 whole cell extract (control) were resuspended in 1X RIPA buffer, separated on a polyacrylamide gel, and transferred to a polyvinylidene difluoride (PVDF) membrane.

The immunoprecipitated proteins were extracted with 2× Laemmli buffer, separated on an SDS-polyacrylamide gel electrophoresis (SDS-PAGE) gel, and the AR band detected by Western blotting.

To determine if rIDE modifies gE, we incubated biotin-labeled gEt protein with rIDE or control proteins (P7.5-Fc which encodes vaccinia virus P7.5 protein or BZLF2-Fc which encodes Epstein-Barr virus BZLF2 protein) at 37°C, 22°C, or 4°C for 30 min. The proteins were then boiled in sample buffer, separated on an SDS-PAGE gel, and gEt was stained with streptavidin conjugated-horse radish peroxidase.

The immunoprecipitates were boiled in SDS loading buffer, separated on an SDS-polyacrylamide gel, and immunoblotted with anti-FLAG M2 mouse mAb, anti-CD98hc goat pAb (N-20) or anti-β galactosidase rabbit pAb.

The immunoprecipitates were boiled in SDS loading buffer, separated on an SDS-polyacrylamide gel, and immunoblotted with anti-CD98hc goat pAb (N-20), anti-β galactosidase rabbit pAb, or anti-integrin β1 rabbit pAb.

Briefly, cells were lysed with radio-immunoprecipitation assay (RIPA) buffer, separated on an 10% sodium dodecyl sulfate (SDS) polyacrylamide gel, and then transferred to polyvinylidene fluoride (PVDF) filters (0.45 μm pore; Millipore, Amherst, MA) by semidry blot using Tris-glycine/20Tris-glycine/20%fer buffer.