After 30 min in 4% paraformaldehyde and washing in phosphate buffer saline, sections were mounted with Dako Glycerol mounting medium or further treated for immunohistochemistry.
After washes in wash buffer and PBS, sections were mounted with Moviol 4 88 (Calbiochem) containing 2.5% 1,4 diazobicyclo-[2,2,2]-octane (DABCO; Sigma) for observation on a Zeiss Axioplan 2 fluorescence microscope equipped with an AxioCam camera and Axio Vision 2.05 software (Carl Zeiss); digital images were processed using Adobe Photoshop version 7.0 (Adobe Systems).
Following reactions with the reagents from an ABC kit (Avidin-Biotin Complex, Vector, CA, USA) as per manufacturer's recommendations, the reaction products were visualized by 3,3 diaminobenzidine, 0.003% H2O2 in 0.5 M TRIS buffer, and pH 7.6 before the sections were mounted on gelatin-coated slides using Permount (Fisher, USA).
Sections were mounted in phosphate buffered saline/glycerol.
After rinsing, the brain sections were mounted on gelatin-coated slides and covered with a cover glass, using glycerol buffer as a mounting medium.
Striatal coronal sections were mounted onto slides and dried prior to rehydration in 0.5 M Tris buffered saline containing 0.05% Tween-20 (TBST, pH7.4).
Finally, all sections were mounted onto gelatine/chrome alum-coated microscope slides and cover-slipped using glycerol in carbonate buffer.
Briefly, brain sections were mounted onto gelatine-coated slides, permeabilized with 0.1% Triton X-100 in phosphate buffered saline (PBS, pH 7.4), and washed with PBS.
Briefly, 5- μm paraffin sections were mounted on poly L-lysine-coated slides for heat-induced epitope retrieval ('HIER' technique) in citrate buffer.
In brief, 5 μm paraffin sections were mounted on poly-L-lysine coated slides for heat-induced epitope retrieval ('HIER' technique) in citrate buffer.
